identicalClones - Identical clustering-based method for partitioning Ig sequences into clones.

Description

The identicalClones function provides a computational pipline for assigning Ig sequences into clonal groups sharing same V gene, J gene, and identical junction.

Usage

identicalClones(
db,
method = c("nt", "aa"),
junction = "junction",
v_call = "v_call",
j_call = "j_call",
clone = "clone_id",
first = FALSE,
cdr3 = FALSE,
mod3 = FALSE,
max_n = 0,
nproc = 1,
verbose = FALSE,
log = NULL,
summarize_clones = TRUE
)

Arguments

db
data.frame containing sequence data.
method
one of the "nt" for nucleotide based clustering or "aa" for amino acid based clustering.
junction
character name of the column containing junction sequences. Also used to determine sequence length for grouping.
v_call
character name of the column containing the V-segment allele calls.
j_call
character name of the column containing the J-segment allele calls.
clone
the output column name containing the clonal clustering identifiers.
first
specifies how to handle multiple V(D)J assignments for initial grouping. If TRUE only the first call of the gene assignments is used. If FALSE the union of ambiguous gene assignments is used to group all sequences with any overlapping gene calls.
cdr3
if TRUE removes 3 nucleotides from both ends of "junction" prior to clustering (converts IMGT junction to CDR3 region). If TRUE this will also remove records with a junction length less than 7 nucleotides.
mod3
if TRUE removes records with a junction length that is not divisible by 3 in nucleotide space.
max_n
The maximum number of N’s to permit in the junction sequence before excluding the record from clonal assignment. Default is set to be zero. Set it as "NULL" for no action.
nproc
number of cores to distribute the function over.
verbose
if TRUE prints out a summary of each step cloning process. if FALSE (default) process cloning silently.
log
output path and filename to save the verbose log. The input file directory is used if path is not specified. The default is NULL for no action.
summarize_clones
if TRUE performs a series of analysis to assess the clonal landscape and returns a ScoperClones object. If FALSE then a modified input db is returned.

Value

If summarize_clones=TRUE (default) a ScoperClones object is returned that includes the clonal assignment summary information and a modified input db in the db slot that contains clonal identifiers in the specified clone column. If summarize_clones=FALSE modified data.frame is returned with clone identifiers in the specified clone column.

Details

identicalClones provides a computational platform to explore the B cell clonal relationships in high-throughput Adaptive Immune Receptor Repertoire sequencing (AIRR-seq) data sets. This function performs clustering among sequences of B cell receptors (BCRs, immunoglobulins, Ig) that share the same V gene, J gene, and identical junction:

Examples

# Find clonal groups
results <- identicalClones(ExampleDb)

# Retrieve modified input data with clonal clustering identifiers
df <- as.data.frame(results)

# Plot clonal summaries 
plot(results, binwidth=0.02)

2

See also

See plotCloneSummary plotting summary results.